@article{APS9342,
author = {Lu GAN and Zhen-hua WANG and Hong ZHANG and Xin ZHOU and Hui ZHOU and Chao SUN and Jing SI and Rong ZHOU and Cheng-jun MA and Ji LI},
title = {Endothelium-independent vasorelaxant effect of 20(S)-protopanaxadiol on isolated rat thoracic aorta},
journal = {Acta Pharmacologica Sinica},
volume = {37},
number = {12},
year = {2017},
keywords = {},
abstract = {Aim: Ginsenosides are considered to be the major pharmacologically active ginseng constituents, whereas 20(S)-protopanaxadiol [20(S)-PPD] is the active metabolite of ginsenosides in gut. In this study we investigated the effect of 20(S)-PPD on isolated rat thoracic aortas as well as its vasorelaxant mechanisms.
Methods: Aortic rings with or without endothelium were prepared from Wistar rats and suspended in organ-chambers. The changes in tension of the preparations were recorded through isometric transducers connected to a data acquisition system. The aortic rings were precontracted with phenylephrine (PE, 1 μmol/L) or high-K+ (80 mmol/L).
Results: Application of 20(S)-PPD (21.5–108.5 μmol/L) caused concentration-dependent vasodilation of endothelium-intact aortic rings precontracted with PE or high-K+, which resulted in the EC50 values of 90.4 or 46.5 μmol/L, respectively. The removal of endothelium had no effect on 20(S)-PPD-induced relaxation. The vasorelaxant effect of 20(S)-PPD was also not influenced by the preincubation with β-adrenergic receptor antagonist propranolol, or with ATP-sensitive K+ channel blocker glibenclamide, voltagedependent K+ channel blocker 4-AP and inward rectifier K+ channel blocker BaCl2, whereas it was significantly attenuated by the preincubation with Ca2+-activated K+ (BKCa) channel blocker TEA (1 mmol/L). Furthermore, the inhibition of NO synthesis, cGMP and prostacyclin pathways did not affect the vasorelaxant effect of 20(S)-PPD. In Ca2+-free solution, 20(S)-PPD (108.5 μmol/L) markedly decreased the extracellular Ca2+-induced contraction in aortic rings precontracted with PE or high-K+ and reduced PE-induced transient contraction. Voltage-dependent Ca2+ channel antagonist nifedipine inhibited PE-induced contraction; further inhibition was observed after the application of receptor-operated Ca2+ channel inhibitor SK&F 96365 or 20(S)-PPD.
Conclusion: 20(S)-PPD induces vasorelaxation via an endothelium-independent pathway. The inhibition of voltage-dependent Ca2+ channels and receptor-operated Ca2+ channels and the activation of Ca2+-activated K+ channels are probably involved in the relaxation.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/9342}
}