@article{APS9237,
author = {Carmen Gonzalez and Hector Rosas-hernandez and Brenda Jurado-manzano and Manuel Alejandro Ramirez-lee and Samuel Salazar-garcia and Pedro Pablo Martinez-cuevas and Aída Jimena Velarde-salcedo and Humberto Moralesloredo and Ricardo Espinosa-tanguma and Syed F Ali and Rafael Rubio},
title = {The prolactin family hormones regulate vascular tone through NO and prostacyclin production in isolated rat aortic rings},
journal = {Acta Pharmacologica Sinica},
volume = {36},
number = {5},
year = {2017},
keywords = {},
abstract = {Aim: Prolactin family hormones include growth hormone, placental lactogen and prolactin, which are able to regulate angiogenesis via NO and prostaglandins. However, their effects on vascular tone are not fully understood. The aim of this study was to evaluate the effects of prolactin family hormones on rat vascular tone in vitro.
Methods: Aortic rings were prepared from adult male rats and precontracted with phenylephrine, then treated with the hormones and drugs. The tension was measured with isometric force displacement transducer connected to a polygraph. NO production and prostacyclin release in physiological solution was determined. Cultured rat aortic endothelial cells (RAECs) were treated with the hormones and drugs, and the phosphorylation of eNOS at serine 1177 was assessed using Western bolt analysis.
Results: Administration of growth hormone or placental lactogen (0.01–100 nmol/L) induced endothelium-dependent vasodilation. Both the hormones significantly increased the phosphorylation of eNOS in RAECs and NO level in physiological solution. Preincubation with L-NAME blocked growth hormone- or placental lactogen-induced vasodilation and NO production. Preincubation with an antibody against growth hormone receptors blocked growth hormone- and placental lactogen-induced vasodilation. Addition of a single dose of prolactin (0.01 nmol/L) induced sustained vessel relaxation, whereas multiple doses of prolactin induced a biphasic contractionrelaxation effect. The vascular effects of prolactin depended on endothelium. Prolactin significantly increased the level of prostacyclin I2 in physiological solution. Preincubation with indomethacin or an antibody against prolactin receptors blocked prolactin-induced vasodilation.
Conclusion: The prolactin family hormones regulate rat vascular tone, selectively promoting either relaxation or contraction of vascular smooth muscle via activation of either growth hormone receptors or prolactin receptors within the endothelium.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/9237}
}