@article{APS9190,
author = {Kang Chen and Bi-zhu Chu and Feng Liu and Bin Li and Chun-mei Gao and Lu-lu Li and Qin-sheng Sun and Zhi-fa Shen and Yu-yang Jiang},
title = {New benzimidazole acridine derivative induces human colon cancer cell apoptosis in vitro via the ROS-JNK signaling pathway},
journal = {Acta Pharmacologica Sinica},
volume = {36},
number = {9},
year = {2017},
keywords = {},
abstract = {Aim: To investigate the mechanisms underlying anticancer action of the benzimidazole acridine derivative N-\{(1H-benzo[d]imidazol-2-yl)methyl\}-2-butylacridin-9-amine(8m) against human colon cancer cells in vitro.
Methods: Human colon cancer cell lines SW480 and HCT116 were incubated in the presence of 8m, and then the cell proliferation and apoptosis were measured. The expression of apoptotic/signaling genes and proteins was detected using RT-PCR and Western blotting. ROS generation and mitochondrial membrane depolarization were visualized with fluorescence microscopy.
Results: 8m dose-dependently suppressed the proliferation of SW480 and HCT116 cells with IC50 values of 6.77 and 3.33 μmol/L, respectively. 8m induced apoptosis of HCT116 cells, accompanied by down-regulation of Bcl-2, up-regulation of death receptor-5 (DR5), truncation of Bid, cleavage of PARP, and activation of caspases (including caspase-8 and caspase-9 as well as the downstream caspases-3 and caspase-7). Moreover, 8m selectively activated JNK and p38 without affecting ERK in HCT116 cells. Knockout of JNK1, but not p38, attenuated 8m-induced apoptosis. In addition, 8m induced ROS production and mitochondrial membrane depolarization in HCT116 cells. Pretreatment with the antioxidants N-acetyl cysteine or glutathione attenuated 8m-induced apoptosis and JNK activation in HCT116 cells.
Conclusion: The new benzimidazole acridine derivative, 8m exerts anticancer activity against human colon cancer cells in vitro by inducing both intrinsic and extrinsic apoptosis pathways via the ROS-JNK1 pathway.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/9190}
}