How to cite item

Effects and mechanisms of melatonin on immune responses in mice of different months

   
@article{APS9185,
	author = {Wei Wei and Yu-Xian Shen and Min Dai and Qun Chen},
	title = {Effects and mechanisms of melatonin on immune responses in mice of different months},
	journal = {Acta Pharmacologica Sinica},
	volume = {24},
	number = {7},
	year = {2016},
	keywords = {},
	abstract = {AIM: To study the effects and mechanisms of melatonin (MT) on immune responses in mice of different months. 
METHODS: Thymocyte proliferation and IL-2 activity were assayed by 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) and activated mouse splenocyte proliferation methods, respectively; cAMP and methionine-enkephalin (met-Enk) level was determined by competitive protein binding assay and radioimmunoassay, respectively. 
RESULTS: The function of lymphocytes, obtained from BALB/c mice aged 6 and 11 months were decreased, which was restored by melatonin at the dose of 5 mg/kg or 30 mg/kg. In vitro, proliferation of lymphocytes in 11-month-old mice was decreased and cAMP level was increased. Melatonin (0.1 nmol/L or 1 micromol/L) had negative regulation to this. Forskolin (10 micromol/L) enhanced the cAMP level of lymphocytes in 2- and 11-month-old mice (P},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/9185}
}