@article{APS9164,
author = {Chen-Xia He and Chao-Yu Miao and Ji-Hua Yao and Hao-Ming Chen and Da-Ru Lu and Ding-Feng Su and Jing-Lun Xue},
title = {Construction of a recombinant vector based on AAV carrying human endothelial nitric-oxide synthase gene},
journal = {Acta Pharmacologica Sinica},
volume = {24},
number = {7},
year = {2016},
keywords = {},
abstract = {AIM: To construct an AAV based vector carrying human endothelial nitric-oxide synthase (eNOS) cDNA and study its expression in vitro for future gene therapy.
METHODS: eNOS cDNA was inserted into the EcoR I site of pSNAV-1 containing the cytomegalovirus (CMV) promoter and inverted terminal repeat sequences of adeno-associated virus. The constructed vector was transfected into BHK and C2C12 cells. eNOS cDNA and mRNA were detected by polymerase chain reaction (PCR) and reverse transcription-PCR (RT-PCR), respectively.
RESULTS: By restriction enzyme digestion analysis, it was proved that eNOS cDNA was inserted into pSNAV-1 in a proper direction. PCR detection demonstrated that pSNAV-eNOS was transferred into both BHK and C2C12 cells. RT-PCR analysis showed that these pSNAV-eNOS transfected cells could express eNOS mRNA.
CONCLUSION: pSNAV-eNOS was successfully constructed with the ability to express human eNOS mRNA in cultured mammalian cells.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/9164}
}