@article{APS9161,
author = {Qing-Guang Dong and Jing-Kai Gu and Da-Fang Zhong and J Paul Fawcett and Hui Zhou},
title = {Metabolism of SFZ-47 in chicken embryo by liquid chromatography-electrospray ion trap mass spectrometry.},
journal = {Acta Pharmacologica Sinica},
volume = {24},
number = {6},
year = {2016},
keywords = {},
abstract = {AIM: To develop an alternative method for investigation of drug metabolism by fertilized chicken eggs using 3H-1,2-dihydro-2-(4-methyl-phenylamino) methyl-1-pyrrolizinone (SFZ-47) as a probe drug. METHODS: SFZ-47 (15 mg) was injected into the albumen of eggs from standardized breed chickens previously incubated for 10 d. After 72 h of further incubation, the allantoic liquid was subjected to solid phase extraction on XAD-2 columns and analyzed by liquid chromatography-electrospray ion trap mass spectrometry method. RESULTS: Three major metabolites were identified, namely 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methyl-amino) benzyl alcohol (SFZ-47-OH), 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methyl-amino)-benzoic acid (SFZ-47-COOH), and its glucuronide conjugates. The metabolic profile was little different from that previously found in rabbits and dogs. CONCLUSION: The result demonstrates the usefulness of the fertilized chicken egg as a convenient source of both phase I and phase II metabolites for further metabolism studies of SFZ-47.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/9161}
}