@article{APS9154,
author = {Yao Song and Yan Huang and Er-Dan Dong and Qi-De Han and You-Yi Zhang},
title = {Characterization of cAMP accumulation mediated by three alpha1-adrenoceptor subtypes in HEK293 cells.},
journal = {Acta Pharmacologica Sinica},
volume = {24},
number = {6},
year = {2016},
keywords = {},
abstract = {AIM: To investigate the characterization of cAMP response mediated by alpha1-adrenoceptor (alpha1-AR) subtypes in HEK293 cells. METHODS: (1) Full-length cDNA encoding three alpha1-AR subtypes were transfected into HEK293 cells by the calcium phosphate precipitation method, respectively. (2) The densities of alpha1-AR subtypes expressed in HEK293 cells were measured by radioligand binding assay. (3) cAMP accumulation was measured by [3H]adenine prelabeling method. RESULTS: (1) Activation of each of three subtypes resulted in an increase of cAMP accumulation in HEK293 cells in a dose-dependent manner, which was inhibited by selective alpha1-AR antagonist prazosin. (2) Comparing the pharmacological property, the maximal responses of alpha1A-AR to agonists were the most potent, while the sensitivity of alpha1-AR subtypes to norepinephrine (NE) was the highest. CONCLUSION: Each of three alpha1-AR subtypes can mediate cAMP accumulation in HEK293 cell line, and there are differences in pharmacological property.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/9154}
}