@article{APS9141,
author = {Hong Zhang and Yong-Yu Li and Sheng-Nian Wang and Kong-Hua Zhang and Xian-Zhong Wu},
title = {Effects of lipopolysaccharides on calcium homeostasis in isolated pancreatic acinar cells of rat},
journal = {Acta Pharmacologica Sinica},
volume = {24},
number = {8},
year = {2016},
keywords = {},
abstract = {AIM: To investigate the effects of lipopolysaccharides (LPS, endotoxin) on the calcium content in pancreatic acinar cells and the origin of Ca2+ during calcium overload induced by LPS, further to explore the mechanism of LPS in inducing calcium overload and pancreatic acinar cell injury.
METHODS: Male rat pancreatic acinar cells were isolated by collagenase digestion and loaded with Fluo-3/AM, then exposed to varying doses of LPS (from 1 mg/L to 20 mg/L). The dynamic change of [Ca2+]i in single pancreatic acinar cell in the absence and presence of Ca2+ in extracellular fluid was determined by laser scanning confocal microscopy. Cell viability was determined by MTT at different time points after treatment with LPS.
RESULTS: Under physiological calcium concentration in extracellular fluid, LPS (10 mg/L) initiated a rapid, concentration-dependent rise in intracellular [Ca2+]i and consequent cell damage (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/9141}
}