@article{APS9134,
author = {Zhen-Lin Zhang and Shui-Xian Shen and Bo Lin and Lu-Yang Yu and Li-Hua Zhu and Wei-Ping Wang and Fei-Hong Luo and Li-He Guo},
title = {Intramuscular injection of interleukin-10 plasmid DNA prevented autoimmune diabetes in mice},
journal = {Acta Pharmacologica Sinica},
volume = {24},
number = {8},
year = {2016},
keywords = {},
abstract = {AIM: To investigate the effect of plasmid coding interleukin-10 (IL-10) DNA on the development of autoimmune diabetes induced by multiple low doses of streptozotocin (STZ) in mice.
METHODS: Injection of STZ (40 mg/kg, i.p.) was given daily for five consecutive days. pcDNA3-IL-10 plasmid (IL-10-treated group) or pcDNA3-null plasmid (pcDNA3-null-treated group) (100 microg DNA once a day) were injected into skeletal muscles of mice on d 1 and d 14. Blood glucose concentration was measured. After mice were killed on d 28, serum IFN-gamma level was measured by ELISA, and pancreatic IL-1beta and TNF-alpha mRNA expression was detected by semi-quantitative reverse-transcription PCR (RT-PCR). The number of CD4+ and CD8+ lymphocytes from spleen was detected using FACS. In addition, pancreatic histology was measured for determination of insulitis grades.
RESULTS: Treatment with pcDNA3-IL-10 resulted in the retention and expression of the vector in skeletal muscle, associated with a considerable elevation in the plasma level of IL-10, which was not observed in pcDNA3-null-treated mice. In IL-10-treated diabetic mice induced by STZ, delay-type hypersensitivity responses were suppressed and the glucose level was greatly lower on d 14, 21, and 28 than pcDNA3-null-treated group (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/9134}
}