@article{APS9132,
author = {Lan-Dian Hu and Guang-Yong Zheng and Hai-Song Jiang and Yu Xia and Yi Zhang and Xiang-Yin Kong},
title = {Mutation analysis of 20 SARS virus genome sequences: evidence for negative selection in replicase ORF1b and spike gene},
journal = {Acta Pharmacologica Sinica},
volume = {24},
number = {8},
year = {2016},
keywords = {},
abstract = {AIM: Recently, more SARS-CoV virus genome sequences are released to the GenBank database. The aim of this study is to reveal the evolution forces of SARS-CoV virus by analyzing the nucleotide mutations in these sequences.
METHODS: We obtained 20 SARS-CoV virus genome sequences from NCBI database, and calculated the ratio of non-synonymous nucleotide substitution per non-synonymous site (Ka) and synonymous nucleotide substitution per synonymous site (Ks) for SARS-CoV virus genes.
RESULTS: The Ka/Ks ratios for replicase polyprotein ORF1a, ORF1b, and spike protein gene are 1.09 (P=0.6501), 0.38 (P=0.0074), 0.65 (P=0.0685) respectively.
CONCLUSION: SARS-CoV virus replicase polyprotein ORF1b is undergoing negative selection; negative selection force is also probably operating on spike protein gene. These results provide basis for future developing a new drug and vaccine against SARS.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/9132}
}