@article{APS9116,
author = {Jian-Hui Liu and Yong-Ming Bao and Ji-Jun Song and Li-Jia An},
title = {Codonopsis pilosula (Franch) Nannf total alkaloids potentiate neurite outgrowth induced by nerve growth factor in PC12 cells},
journal = {Acta Pharmacologica Sinica},
volume = {24},
number = {9},
year = {2016},
keywords = {},
abstract = {AIM: To explore the effect of Codonopsis pilosula (Franch) Nannf total alkaloids (DSA) on differentiation induced by nerve growth factor (NGF) in PC12 cells.
METHODS: After culturing PC12 cells with DSA in the presence or absence of NGF, neurite outgrowth in PC12 cells and correlated protein kinases were assayed.
RESULTS: DSA alone did not exhibit neuritogenic activity, but caused a significant enhancement of NGF (2 microg/L)-induced neurite outgrowth in PC12 cells, and increased the phosphorylation of mitogen-activated protein kinase (MAPK). Furthermore, this enhancing effect was completely blocked by a specific MAPK kinase inhibitor, PD98059.
CONCLUSION: DSA enhanced the NGF-induced neurite outgrowth in PC12 cells by amplifying an up-stream step of the MAPK-dependent signaling pathway.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/9116}
}