@article{APS9114,
author = {Li Zhang and Xiao-Dong Liu and Lin Xie and Guang-Ji Wang},
title = {P-glycoprotein restricted transport of nimodipine across blood-brain barrier},
journal = {Acta Pharmacologica Sinica},
volume = {24},
number = {9},
year = {2016},
keywords = {},
abstract = {AIM: To examine whether the transport of nimodipine (NMD) from the circulating blood into the brain is restricted by P-glycoprotein (P-gp) in rat brain capillary endothelial cells (BCEC).
METHODS: When cells reached confluence, a time course of NMD uptake was recorded by incubation with a medium containing NMD 10 mg/L at 37 degrees C. Effects of various agents in the steady-state uptake of NMD were tested by co-administration with NMD and each compound to cells at 37 degrees C for 90 min. The uptake of NMD was measured for 90 min. Effects of P-gp inhibitors on the efflux of NMD from primary cultured BCEC were studied by administration of erythromycin, clarithromycin, cyclosporin A (CsA), and Hanks' solution after the accumulation of NMD by BCEC at 37 degrees C for 90 min.
RESULTS: The uptake of NMD by primary cultured rat BCEC was time-dependent, and the steady-state uptake of NMD was increased in the presence of several substrates of P-gp in BCEC. The steady-state uptake was also significantly increased (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/9114}
}