@article{APS9112,
author = {Guo-Hua Zhang and Xiao-Fan Tan and Dong Shen and Shu-Yuan Zhao and Yan-Yi Shi and Cai-Ke Jin and Wei-Gu Sun and Yan-Hong Guo and Kuang-Hueih Chen and Jian Tang},
title = {Gene expression and antitumor effect following im electroporation delivery of human interferon alpha 2 gene},
journal = {Acta Pharmacologica Sinica},
volume = {24},
number = {9},
year = {2016},
keywords = {},
abstract = {AIM: To investigate the gene expression and antitumor effect following im electroporation delivery of human interferon alpha 2 (hIFN-alpha 2) gene.
METHODS: The pcD2/hIFN-alpha 2 was injected into the middle of the quadriceps muscle of female BALB/c mice or the leukemia-bearing female BALB/c nude mice, and then electroporation was given to the injection site. Optimal electrical parameters and the efficiency of gene transfer was studied with hIFN-alpha 2 ELISA kit. The HL-60 tumor model in BALB/c nude mice was used to investigate therapeutic effects of im electroporation delivery of pcD2/hIFN-alpha 2.
RESULTS: The optimal conditions for the electric pulses were as follows: voltage at 200 V/cm; pulse duration at 40 ms per pulse; number of pulse at 6 pulses and frequency at 1 Hz. Under optimal conditions, the serum hIFN-alpha 2 levels in electroporation group (160 microg/L+/-31 microg/L) were 45-fold higher than those of nonelectroporation group (3.6 microg/L+/-1.6 microg/L, P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/9112}
}