@article{APS9111,
author = {Zhen-Lin Zhang and Bo Lin and Lu-Yang Yu and Shui-Xian Shen and Li-Hua Zhu and Wei-Ping Wang and Li-He Guo},
title = {Gene therapy of experimental autoimmune thyroiditis mice by in vivo administration of plasmid DNA coding for human interleukin-10},
journal = {Acta Pharmacologica Sinica},
volume = {24},
number = {9},
year = {2016},
keywords = {},
abstract = {AIM: To investigate the effect of interleukin-10 (IL-10) gene on experimental autoimmune thyroiditis mice.
METHODS: Mice were immunized to induce autoimmune thyroiditis with porcine thyroglobulin (pTg), and thyroids of mice were injected with IL-10 DNA. On d 28 after immunization with pTg, mRNA expression of IL-10 in thyroid glands was detected and thyroid specimens were histopathological studied.
RESULTS: The mRNA expression of IL-10 was detected in thyroid glands on d 7 and 14 after injection of IL-10 plasmid DNA or on COS-7 cells 48 h after IL-10 plasmid DNA transfection. In addition, hIL-10 levels in culture media significantly increased 48 h and 72 h after IL-10 plasmid DNA transfection. Infiltration index of lymphocytes (1.1+/-0.4) in thyroids of IL-10-treated mice was significantly lower than that of pcDNA3-null-treated mice (2.2+/-0.5) (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/9111}
}