@article{APS9109,
author = {Yan-Ling Yu and Peng-Yuan Yang and Hui-Zhi Fan and Zhen-Yu Huang and Yao-Cheng Rui and Peng-Yuan Yang},
title = {Protein expressions in macrophage-derived foam cells: comparative analysis by two-dimensional gel electrophoresis},
journal = {Acta Pharmacologica Sinica},
volume = {24},
number = {9},
year = {2016},
keywords = {},
abstract = {AIM: To study the overall protein expression changes induced by oxidized low-density lipoprotein (ox-LDL) in the U937 foam cells.
METHODS: Foam cell model was established by incubating the human monoblastic leukemia (U937) cells with ox-LDL. Each protein samples in U937 control cells and U937 foam cells were separated by two-dimensional gel electrophoresis (2-DE). After the gels were stained by silver staining method, the images were analyzed by PDquest 2D-image-analysis software (Bio-Rad). Some of the spots were available via the Internet with links to the U937 proteomic map provided from the ExPASy Proteomics server.
RESULTS: Using 2-DE, the overall protein map was obtained, in which 150 spots were matched with the control gel (match ratio: 75 %). Compared with U937 cells, 37 spots significantly changed in the foam cells (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/9109}
}