@article{APS9069,
author = {Kang Yao and Biao Xu and Ya-Ping Liu and Albert Ferro},
title = {Effects of beta-adrenoceptor stimulation on endothelial nitric-oxide synthase phosphorylation of human umbilical vein endothelial cells.},
journal = {Acta Pharmacologica Sinica},
volume = {24},
number = {3},
year = {2016},
keywords = {},
abstract = {AIM: To investigate the effects of isoprenaline on the phosphorylation level of endothelial nitric oxide synthase (eNOS) of human umbilical vein endothelial cells (HUVEC) and to elucidate the regulation mechanisms of beta-adrenoceptor (beta-AR) on eNOS activity. METHODS: HUVEC were exposed to isoprenaline (1 micromol/L) for 30 min, in the absence or presence of selective beta1- or beta2 -adrenoceptor antagonists. eNOS was then isolated by immunoprecipitation using a specific eNOS antibody. eNOS activity was determined by the conversion of L-[3H]arginine to L-[3H]citrulline. The levels of eNOS expression and eNOS phosphorylation were measured by Western blotting. RESULTS: Treatment of HUVEC with isoprenaline (1 micromol/L) for 30 min caused no measureable change in eNOS expression. However, eNOS activity and serine phosphorylation level were significantly increased (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/9069}
}