@article{APS9052,
author = {Feng Xu and Yong-Su Zhen},
title = {(-)-Epigallocatechin-3-gallate enhances anti-tumor effect of cytosine arabinoside on HL-60 cells.},
journal = {Acta Pharmacologica Sinica},
volume = {24},
number = {2},
year = {2016},
keywords = {},
abstract = {AIM: To study the potentiation of anti-tumor effect induced by cytosine arabinoside (AraC) with (-)-epigallocatechin-3-gallate (EGCG). METHODS: Growth curve method and MTT assay were used to measure the cytotoxic effect of AraC alone or in combination with EGCG on HL-60 cells. Flow cytometry was used to study the cell cycle distribution of HL-60 cells. Nullification assay was used to examine whether EGCG would nullify the rescue effect of deoxycytidine (dCdR) to AraC. Western blot analysis was employed to investigate bcl-2 expression. Intracellular Ca2+ assay was evaluated. RESULTS: Inhibition of HL-60 cell proliferation induced by AraC was enhanced by EGCG, with multiplication time prolonging from 48 h to 70 h and growth saturation density decreasing from 5.78 to 5.54. The MTT results indicated that IC50 was decreased from (0.34+/-0.29) micromol/L (AraC alone) to (0.11+/-0.09) micromol/L (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/9052}
}