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Antisense Sp1 oligodeoxynucleotide decreases telomerase activity by inhibiting hTERT mRNA expression in Jurkat T cells.

   
@article{APS9030,
	author = {Jian-Xin Pang and Xi-Yuan Cheng and Wei Xu and Shu-Guang Wu},
	title = {Antisense Sp1 oligodeoxynucleotide decreases telomerase activity by inhibiting hTERT mRNA expression in Jurkat T cells.},
	journal = {Acta Pharmacologica Sinica},
	volume = {24},
	number = {1},
	year = {2016},
	keywords = {},
	abstract = {AIM: To study the effects of transcriptional factor Sp1 antisense oligodeoxynucleotide (ODN) on telomerase activity and human telomerase reverse transcriptase (hTERT) expression. METHODS: Antisense oligodeoxynucleotide (ODN) was designed to inhibit Sp1 expression and transferred to Jurkat T cells by lipofectamin. Telomerase PCR-ELISA was used to detect telomerase activity. RT-PCR analysis was used to assess the mRNA expression of Sp1 and hTERT, and Western blot was used to analyze the levels of Sp1 protein. RESULTS: Treatment of Jurkat T cells with Sp1 antisense ODN (1 micromol/L) dramatically reduced Sp1 mRNA and protein levels. The inhibition rate was 44.8 % (P },
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/9030}
}