@article{APS9012,
author = {Li-Ying Fu and Fang Wang and Xue-Song Chen and Hong-Yi Zhou and Wei-Xing Yao and Guo-Jin Xia and Ming-Xing Jiang},
title = {Perforated patch recording of L-type calcium current with beta-escin in guinea pig ventricular myocytes},
journal = {Acta Pharmacologica Sinica},
volume = {24},
number = {11},
year = {2016},
keywords = {},
abstract = {AIM: To establish a perforated patch recording (PPR) mode with beta-escin and compare L-type calcium current (I(Ca,L)) recorded under PPR and normal whole-cell recording (WCR) condition in isolated guinea-pig ventricular myocytes.
METHODS: Single myocytes were dissociated by enzymatic dissociation method. beta-escin was added to the pipette solution to perforate the cell membrane and obtain PPR mode. I(Ca,L) was recorded using PPR and WCR techniques.
RESULTS: beta-Escin 20, 25, and 30 micromol/L could permeabilize the cell membrane and obtain PPR mode. With beta-escin 25 micromol/L, the success rate was highest (16/17, 94 %) and the time required for permibilization was 2-15 (8+/-4) min. Run-down of I(Ca,L) was considerably slower in PPR than in WCR condition. The amplitude of I(Ca,L) was decreased by 36 % at 20 min after the formation of WCR, while it was slowly decreased by 8 % at 30 min after the formation of PPR. The current-voltage relation (I-V) curves, activation and inactivation curves of I(Ca,L) were not significantly different between WCR and PPR. The inactivation rate of ICa,L was slower in PPR than in WCR, the faster inactivation time constant (tau(f)) was longer in PPR than in WCR at membrane potentials of -20 mV -- +10 mV (n=6, P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/9012}
}