@article{APS8992,
author = {Ben-Bo Gao and Qi-De Han},
title = {No functional beta 3-adrenergic receptors expressed in rat skeletal muscle cells},
journal = {Acta Pharmacologica Sinica},
volume = {19},
number = {6},
year = {2016},
keywords = {},
abstract = {AIM: To determine the functional role of beta 3-adrenoceptors (beta 3-AR) in rat skeletal muscle cells.
METHODS: Nonselective beta-AR agonist isoprenaline (isoproterenol, Iso), beta 3-AR agonist CGP12177A which is a beta 1-/beta 2-AR antagonist and selective beta 3-AR antagonist SR59230A on cAMP accumulation was studied in primary cultured rat skeletal muscle cells.
RESULTS: Iso stimulated cAMP accumulation in a concentration-dependent manner with EC50 of 1.51 nmol.L-1 and propranolol inhibited cAMP accumulation stimulated by Iso with KB of 3.47 nmol.L-1. CGP12177A had no effect on cAMP accumulation but inhibited cAMP production induced by Iso. SR59230A 10 nmol.L-1 did not inhibit cAMP production induced by Iso.
CONCLUSION: The functional beta 3-AR are not present or at least not coupled to adenylyl cyclase activity in skeletal muscle cells.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8992}
}