@article{APS8947,
author = {Bao-Chang Cai and Tian-Shan Wang and Masahiko Kurokawa and Kimiyasu Shiraki and Masao Hattori},
title = {Cytotoxicities of alkaloids from processed and unprocessed seeds of Strychnos nux-vomica.},
journal = {Acta Pharmacologica Sinica},
volume = {19},
number = {5},
year = {2016},
keywords = {},
abstract = {AIM: To examine the cytotoxicities of 6 crude Strychnos alkaloid fractions from the seeds of Strychnos nux-vomica unprocessed or processed with various traditional processing methods and 13 pure Strychnos alkaloids from the fractions. METHODS: Using cell culture, their inhibitory effects on Vero cell growth-inhibition assay, and host cell DNA synthesis by [3H]thymidine ([3H]TdR) uptake assay. RESULTS: The IC50 of processed seeds were 155% and 212% of unprocessed ones in cell growth-inhibition assay and in [3H]TdR uptake assay, respectively. The IC50 of 13 compounds were 0.45-0.80 mmol.L-1 and 0.50-12 mmol.L-1, respectively. The processing method with sand bath exhibited a wide safety margin compared with other traditional processing methods or no processing. The isomers of Strychnos alkaloids and their N-oxides showed much lower cytotoxicities among these alkaloids. Isobrucine N-oxide showed the lowest cytotoxicity. The contents of isomers and N-oxides of Strychnos alkaloids were the highest in the sand processing. CONCLUSION: Processing of nux vomica plays a critical role in its toxicity.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8947}
}