@article{APS8872,
author = {Tian-Le Xu and Zhi-Ping Pang and Jie-Fang Kang and Ji-Shuo Li},
title = {Isolation of rat sacral dorsal commissural neurons},
journal = {Acta Pharmacologica Sinica},
volume = {19},
number = {3},
year = {2016},
keywords = {},
abstract = {AIM: To isolate rat sacral dorsal commissural neurons (SDCN).
METHODS: Using enzymatic and mechanical dissociation techniques to isolate the neurons and using nystatin perforated patch technique to evaluate their functional state.
RESULTS: The isolated neurons exhibited good responses to excitatory and inhibitory amino acids. The responses of SDCN to N-methyl-D-aspartate were markedly potentiated by substance P and trans-1-aminocyclopentane-1,3-dicarboxylate, whereas GABA responses were significantly potentiated by diazepam, pregnenolone, and pentobarbital.
CONCLUSION: This preparation provides a satisfactory model for exploring the mechanisms of the SDCN in nociception and antinociception.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8872}
}