@article{APS8853,
author = {Kai-Yu Jiang and Chang-Geng Ruan and Zhen-Lun Gu and Wen-Xuan Zhou and Ci-Yi Guo},
title = {Effects of tanshinone II-A sulfonate on adhesion molecule expression of endothelial cells and platelets in vitro},
journal = {Acta Pharmacologica Sinica},
volume = {19},
number = {1},
year = {2016},
keywords = {},
abstract = {AIM: To study the action of tanshinone II-A sulfonate (Tan) on adhesion molecule expression by cultured endothelial cells and platelets.
METHODS: Tumor necrosis factor alpha (TNF-alpha)-induced ICAM-1 expression on the cell surface and endothelial adhesivity toward HL-60 cells were studied using human umbilical vein endothelial cells (HUVEC). Thrombin-induced expression of platelet P-selectin was studied using human blood platelets. Adhesion molecule expression on the cell surface was measured by flow cytometry. The number of HL-60 cells adhering to the HUVEC monolayer was determined by liquid scintillation spectroscopy.
RESULTS: Pretreatment of HUVEC with TNF-alpha significantly enhanced ICAM-1 expression and increased HL-60 cells adhesion to HUVEC from 4.6% +/- 0.7% to 30% +/- 6%. Tan (25-200 mumol.L-1) inhibited the effects of TNF-alpha in a concentration-dependent manner. Tan also inhibited the increase of P-selectin expression of thrombin-activated platelets in a concentration-dependent manner.
CONCLUSION: Tan inhibited expression of adhesion molecules (ICAM-1, P-selectin) in HUVEC and in human blood platelets.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8853}
}