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Increased heart microsomal Na(+) K(+)-transporting ATPase activity by tetrandrine in spontaneously hypertensive rats

  
@article{APS8778,
	author = {Nian-Hang Chen and You-Lin Wang and Jian-Hua Ding},
	title = {Increased heart microsomal Na(+) K(+)-transporting ATPase activity by tetrandrine in spontaneously hypertensive rats},
	journal = {Acta Pharmacologica Sinica},
	volume = {14},
	number = {4},
	year = {2016},
	keywords = {},
	abstract = {In spontaneously hypertensive rats (SHR), the heart microsomal Na+, K(+)-ATPase showed a reduced activity with a higher Ca2+ sensitivity and lower responsiveness to K+. After SHR were treated with tetrandrine (Tet, ig 30 mg.kg-1, qd x 30 d) or when the microsomes obtained from SHR hearts were incubated with various concentrations of Tet, the myocardial Na+,K(+)-ATPase showed a higher activity with a decreased Km for K+, or ATP and an increased Vmax for K+. The in vitro stimulatory effect of Tet on the enzyme was dependent on the Na+ concentration in medium and was not abolished by CaCl2 1 mmol.L-1 or ouabain 0.1 mmol.L-1. However, when given in vivo, Tet tended to reduce the tolerance of the enzyme to Ca2+ and failed to change the responsiveness of the enzyme to ouabain. In vitro, Tet had similar but weaker stimulatory effect on the Na+,K(+)-ATPase from Wistar Kyoto rats.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/8778}
}