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Effects of protein kinase C activator and inhibitor on release of tumor necrosisfactor from mouse peritoneal macrophages

   
@article{APS8738,
	author = {Zhen-Lin Hu and Ding-Hua Qian},
	title = {Effects of protein kinase C activator and inhibitor on release of tumor necrosisfactor from mouse peritoneal macrophages},
	journal = {Acta Pharmacologica Sinica},
	volume = {14},
	number = {2},
	year = {2016},
	keywords = {},
	abstract = {The effects of protein kinase C (PKC) activator 1-O-tetradecanoylphorbol-13-acetate (TPA) and inhibitor 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7) and quercetin were studied on release of tumor necrosis factor (TNF) from mouse peritoneal macrophages primed with Propionibacterium acnes (PA). The results showed that TPA (1-100 ng.ml-1) and lipopolysaccharides (LPS) (1-100 ng.ml-1) induced the release of TNF from PA-primed mouse peritoneal macrophages in dose- and time-dependent manners in vitro, and the effects of TPA and LPS were inhibited by H-7 (12.5-100 mumol.L-1) or quercetin (6.25-25 mumol.L-1) in a dose-dependent manner. After ip H-7 (50 mg.kg-1), LPS-induced release of TNF in vivo decreased significantly. These results suggest that PKC may play a critical role in release of TNF from PA-primed macrophages.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/8738}
}