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Pharmacokinetic studies on 5-fluorouracil and its metabolite in rabbits by high pressure liquid chromatographyi

  
@article{APS8623,
	author = {Bin Zhao and Xiang-Lan Zhao},
	title = {Pharmacokinetic  studies  on  5-fluorouracil and its metabolite in rabbits by high pressure liquid chromatographyi},
	journal = {Acta Pharmacologica Sinica},
	volume = {9},
	number = {3},
	year = {2016},
	keywords = {},
	abstract = {A simple and specific HPLC method was developed to determine simultaneously  5-fluorouracil (5-FU) and its metabolite, 5-fluoro-2'-deoxyuridine( FUdR), in serum. 5-Bromouracil (5-BU) was added as an internal standard before extraction with 16% isopropanol in ethyl acetate. The extracts were then chromatographed using a mobile phase of water at a flow rate of 2  ml/min. Detection was made at 270 nm. The peak height ratias of 5-FU or FUdR  to 5-BU were measured. After single iv doses to rabbits, pharmacokinetic parameters were compared between 6 normal white New Zealand rabbits and 6 hybrid rabbits. The A. B, Kio and Vi values were found to be significantly different between these 2 kinds of rabbits.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/8623}
}