@article{APS8452,
author = {Cui Chen and Xin-yi Mu and Yue Zhou and Ke Shun and Shan Geng and Jun Liu and Jian-wei Wang and Jie Chen and Tin-yu Li and Ya-ping Wang},
title = {Ginsenoside Rg1 enhances the resistance of hematopoietic stem/progenitor cells to radiation-induced aging in mice},
journal = {Acta Pharmacologica Sinica},
volume = {35},
number = {1},
year = {2016},
keywords = {},
abstract = {Cui CHEN1, Xin-yi MU1, Yue ZHOU3, Ke SHUN1, Shan GENG1, Jun LIU1, Jian-wei WANG1, Jie CHEN2, Tin-yu LI2, Ya-ping WANG1, *
1Laboratory of Stem Cells and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China; 2Chongqing Stem Cell Therapy Engineering Technical Center, Chongqing 400016, China; 3Department of Histology and Embryology, Dali University, Dali 671000, China
Aim: To investigate the effects of ginsenoside Rg1 on the radiation-induced aging of hematopoietic stem/progenitor cells (HSC/HPCs) in mice and the underlying mechanisms.
Methods: Male C57BL/6 mice were treated with ginsenoside Rg1 (20 mg·kg-1·d-1, ip) or normal saline (NS) for 7 d, followed by exposure to 6.5 Gy X-ray total body irradiation. A sham-irradiated group was treated with NS but without irradiation. Sca-1+ HSC/HPCs were isolated and purified from their bone marrow using MACS. DNA damage was detected on d 1. The changes of anti-oxidative activities, senescence-related markers senescence-associated β-galactosidase (SA-β-gal) and mixed colony-forming unit (CFU-mix), P16INK4a and P21Cip1/Waf1 expression on d 7, and cell cycle were examined on d 1, d 3, and d 7.
Results: The irradiation caused dramatic reduction in the number of Sca-1+ HSC/HPCs on d 1 and the number barely recovered until d 7 compared to the sham-irradiated group. The irradiation significantly decreased SOD activity, increased MDA contents and caused DNA damage in Sca-1+ HSC/HPCs. Moreover, the irradiation significantly increased SA-β-gal staining, reduced CFU-mix forming, increased the expression of P16INK4a and P21Cip1/Waf1 in the core positions of the cellular senescence signaling pathways and caused G1 phase arrest of Sca-1+ HSC/HPCs. Administration of ginsenoside Rg1 caused small, but significant recovery in the number of Sca-1+ HSC/HPCs on d 3 and d 7. Furthermore, ginsenoside Rg1 significantly attenuated all the irradiation-induced changes in Sca-1+ HSC/HPCs, including oxidative stress reaction, DNA damage, senescence-related markers and cellular senescence signaling pathways and cell cycle, etc.
Conclusion: Administration of ginsenoside Rg1 enhances the resistance of HSC/HPCs to ionizing radiation-induced senescence in mice by inhibiting the oxidative stress reaction, reducing DNA damage, and regulating the cell cycle.
Keywords: ginsenoside Rg1; hematopoietic stem and progenitor cell (HSC/HPC); ionizing radiation; cellular senescence; oxidative stress; DNA damage; cell cycle
This study was supported by the National Natural Science Foundation of China, No 81173398, 30973818, 30970872, and 81202785.
* To whom correspondence should be addressed.
E-mail ypwangcq@aliyun.com
Received 2013-05-05 Accepted 2013-08-28},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8452}
}