@article{APS8450,
author = {Wei Yan and Ting-yu Wang and Qi-ming Fan and Lin Du and Jia-ke Xu and Zan-jing Zhai and Hao-wei Li and Ting-ting Tang},
title = {Plumbagin attenuates cancer cell growth and osteoclast formation in the bone microenvironment of mice},
journal = {Acta Pharmacologica Sinica},
volume = {35},
number = {1},
year = {2016},
keywords = {},
abstract = {Wei YAN1, 3, #, Ting-yu WANG2, #, *, Qi-ming FAN1, Lin Du1, Jia-ke XU4, Zan-jing ZHAI1, Hao-wei LI1, Ting-ting TANG1, *
1Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; 2Department of Pharmacy, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; 3Wendeng Zhenggu Hospital of Shandong Province, Wendeng 264400, China; 4School of Pathology and Laboratory Medicine, University of Western Australia, Nedlands, 6009, Western Australia
Aim: To investigate the effects of plumbagin, a naphthoquinone derived from the medicinal plant Plumbago zeylanica, on human breast cancer cell growth and the cancer cell-induced osteolysis in the bone microenvironment of mice.
Methods: Human breast cancer cell subline MDA-MB-231SA with the ability to spread and grow in the bone was tested. The cell proliferation was determined using the CCK-8 assay. Apoptosis was detected with Annexin V/PI double-labeled flow cytometry. Red fluorescent protein-labeled MDA-MB-231SArfp cells were injected into the right tibia of female BALB/c-nu/nu mice. Three days after the inoculation, the mice were injected with plumbagin (2, 4, or 6 mg/kg, ip) 5 times per week for 7 weeks. The growth of the tumor cells was monitored using an in vivo imaging system. After the mice were sacrificed, the hind limbs were removed for radiographic and histological analyses.
Results: Plumbagin (2.5–20 μmol/L) concentration-dependently inhibited the cell viability and induced apoptosis of MDA-MB-231SA cells in vitro (the IC50 value of inhibition of cell viability was 14.7 μmol/L). Administration of plumbagin to breast cancer bearing mice delayed the tumor growth by 2–3 weeks and reduced the tumor volume by 44%–74%. The in vivo imaging study showed that plumbagin dose-dependently inhibited MDA-MB-231SArfp cell growth in bone microenvironment. Furthermore, X-ray images and micro-CT study demonstrated that plumbagin reduced bone erosion area and prevented a decrease in bone tissue volume. Histological studies showed that plumbagin dose-dependently inhibited the breast cancer cell growth, enhanced the cell apoptosis and reduced the number of TRAcP-positive osteoclasts.
Conclusion: Plumbagin inhibits the cell growth and induces apoptosis in human breast cancer cells in mice bone microenvironment, leading to significant reduction in osteolytic lesions caused by the tumor cells.
Keywords: plumbagin; naphthoquinone; breast cancer; lytic lesion; osteoclast; bone microenvironment; apoptosis; in vivo imaging
This work was supported by the grants from the National Natural Science Foundation of China (No 81172549, 81228013 and 81301531), the Shanghai Science and Technology Development Fund (No 11XD1403300, 12140901300), the Program of Key Disciplines of Shanghai Municipal Education Commission (No J50206), and the Opening Project of Shanghai Key Laboratory of Orthopaedic Implant (KFKT2011003). The authors would like to thank Dr John PRICE for providing the MDA-MB-231SArfp cell line, which was originally published in Cancer Research 65, 4929, 2005.
# These authors contributed equally to this work.
* To whom correspondence should be addressed.
E-mail ttt@sjtu.edu.cn (Ting-ting TANG); wangty1982@gmail.com (Ting-yu WANG)
Received 2013-07-25 Accepted 2013-09-18},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8450}
}