@article{APS8431,
author = {Jiong YANG and Wei GUO and Han-xiang NIE and Su-ping HU},
title = {Interleukin-12 was not involved in promotion of T helper cell differentiation induced by theophylline},
journal = {Acta Pharmacologica Sinica},
volume = {25},
number = {12},
year = {2016},
keywords = {},
abstract = {AIM:
To investigate the effect of theophylline on the naive T cell differentiation and the probable role of interleukin-12 (IL-12).
METHODS:
Naive cord blood T cells were treated with theophylline 10 mg/L for 3 d after stimulation with PHA 100 mg/L. Differentiation of T cells was analyzed by flow cytometry. Theophylline 10 mg/L and IL-12-mAb 0.025 mg/L were added in cord blood mononuclear cell (CBMC) cultures primed with LPS 1 mg/L to detect the levels of IL-12 and IL-12P40. The whole blood cultures were obtained from twelve health volunteers with or without administration of theophylline (200 mg). Cytokines were measured by enzyme linked immuno-sorbent assay.
RESULTS:
Theophylline promoted T helper 1 (Th1) cells differentiation from naive T cells (21.9 %+/-10.3 % vs 9.4 %+/-5.6 %, P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8431}
}