@article{APS8428,
author = {Hong XIE and Ke-ying CHEN and Pei-hong ZHU},
title = {Effect of Zn2+ ions on ryanodine binding to sarcoplasmic reticulum of striated muscles in the presence of pyrithione},
journal = {Acta Pharmacologica Sinica},
volume = {25},
number = {12},
year = {2016},
keywords = {},
abstract = {AIM:
To explore whether the differential effects of Zn2+ on ryanodine binding to the sarcoplasmic reticulum (SR) of skeletal and cardiac muscles resulted from different permeability of the SR to Zn2+.
METHODS:
[3H]ryanodine binding assays were performed to examine the effect of Zn2+ on ryanodine binding to the SR in the presence of pyrithione sodium (PyNa), a specific Zn2+ ionophore.
RESULTS:
As a control, PyNa up to 50 micromol/L did not induce any effect on ryanodine binding to the SR of cardiac muscle. But PyNa 1-100 micromol/L increased ryanodine binding in skeletal muscle with maximum binding (222.2+/-20.9 % of the control) and inhibited ryanodine binding to 50 % of the control at about 500 micromol/L. In the presence of PyNa 10 and 50 micromol/L the dose-dependence of the effect of Zn2+ in cardiac muscle was still monophasic and not changed by PyNa, while the biphasic effect of Zn2+ in skeletal muscle became monophasic.
CONCLUSION:
Different permeability of the SR to Zn2+ may account for the differential effects of Zn2+ on ryanodine binding in skeletal and cardiac muscles. PyNa is not a strictly specific Zn2+ ionophore.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8428}
}