@article{APS8410,
author = {Li GU and Yuan-jue ZHU and Zi-jian GUO and Xing-xiang XU and Wen-bing XU},
title = {Effect of IFN-gamma and dexamethasone on TGF-beta1-induced human fetal lung fibroblast-myofibroblast differentiation},
journal = {Acta Pharmacologica Sinica},
volume = {25},
number = {11},
year = {2016},
keywords = {},
abstract = {AIM:
To study whether Smads signaling pathway was involved in human fetal lung fibroblast-myofibroblast differentiation induced by transforming growth factor (TGF)-beta1 and the role of interferon (IFN)-gamma, dexamethasone (DEX) in the fibroblast-myofibroblast differentiation.
METHODS:
Alpha-smooth muscle actin (alpha-SMA), Smad2/3, and Smad7 protein were assessed by Western blot. Collagen protein was analyzed by measuring hydroxyproline. Alpha-SMA and collagen III mRNA were assessed by RT-PCR. Myofibroblasts morphology and Smad2/3 nuclear translocation were assessed by immunohistochemistry. The overexpression of Smad7, a negative mediator of Smads signaling pathway, was acquired by transfection of Smad7 vector.
RESULTS:
During fibroblast-myofibroblast differentiation induced by TGF-beta1, IFN-gamma 200 microg/L markedly blocked TGF-beta1-induced alpha-SMA protein expression (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8410}
}