@article{APS8383,
author = {Ming ZHENG and Rong HOU and Rui-ping XIAO},
title = {Acidosis-induced p38 MAPK activation and its implication in regulation of cardiac contractility},
journal = {Acta Pharmacologica Sinica},
volume = {25},
number = {10},
year = {2016},
keywords = {},
abstract = {AIM:
To determine the possible role of pH in mediating activation of p38 mitogen-activated protein kinase (MAPK) and the consequent function of activated p38 MAPK in regulating cardiac contractility.
METHODS:
Adult rat cardiomyocytes were isolated and cultured. Low pH media was used to induce intracellular acidosis and contraction of single cardiomyocyte was measured.
RESULTS:
Phosphorylation of p38 MAPK was increased during ischemia, and pHi was decreased. Intracellular acidosis activated p38 MAPK to a similar level as ischemia. Inhibition of p38 MAPK activation by SB203580, a specific inhibitor of p38 MAPK, reversed acidosis-mediated reduction of myocyte contractility.
CONCLUSION:
In adult rat cardiomyocytes, intracellular acidification activated p38 MAPK and decreased cardiac contractility. Pretreatment of cardiomyocytes with SB203580 completely blocked p38 MAPK activation and partially reversed acidosis-mediated decline of cardiac contractility.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8383}
}