@article{APS8331,
author = {Qi-huang JIN and Heng-yi HE and Yu-fang SHI and He LU and Xue-jun ZHANG},
title = {Overexpression of acetylcholinesterase inhibited cell proliferation and promoted apoptosis in NRK cells},
journal = {Acta Pharmacologica Sinica},
volume = {25},
number = {8},
year = {2016},
keywords = {},
abstract = {AIM:
To study the potential function of acetylcholinesterase (AChE) in apoptosis through overexpression of AChE in Normal Rat Kidney (NRK) cells.
METHODS:
AChE activity was detected by the method of Karnovsky and Roots. Activated caspase-3 was analyzed by Western blotting and immunofluorescence with antibody special to activated caspase-3 fragment. The expression plasmids were constructed in pcDNA3.1 containing AChE gene or a fragment of AChE antisense that were got from RT-PCR. Stable expression cell lines were selected by G418 in cells transfected by lipofection. AChE expression was analyzed by RT-PCR and Western blotting. The proliferation rates of transfected cells were examined by the growth curve and cloning efficiency. MTT assay was used to analyze the cell viability.
RESULTS:
The proliferation rate of the cells transfected with AChE was retarded and the cloning efficiency was lower (28.2 %+/-3.1 % and 48.7 %+/-2.1 %) than cells transfected with vector (56.1 %+/-0.3 %) or AChE-antisense (77.7 %+/-2.2 %). After 2 d the various clone types were deprived of serum, the residue cell viability were 10.4 %+/-4.6 % and 12.6 %+/-6.7 % in the cells transfected with AChE, and 27.4 %+/-3.5 % in cells with vector, and 50.3 %+/-7.8 % in cells with AChE-antisense.
CONCLUSION:
During apoptosis, increase of AChE protein is to inhibit cell proliferation, and then to promote apoptosis in NRK cells.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8331}
}