@article{APS8288,
author = {Zheng-guang Zhu and Shu-guang Wu},
title = {Fibrinogenolytic properties of natrahagin (a proteinase from cobra venom) and its effect on human platelet aggregation.},
journal = {Acta Pharmacologica Sinica},
volume = {20},
number = {10},
year = {2016},
keywords = {},
abstract = {AIM:
To study the fibrinogenolytic properties of natrahagin and its effect on platelet aggregation.
METHOD:
SDS-PAGE, fibrinogenolytic activity assay, platelet aggregation.
RESULTS:
Upon incubation of fibrinogen with natrahagin at the ratio of 50:1 (w/w), A alpha-chains of fibrinogen were almost completely hydrolyzed in 5 min; however, at least 6 h was needed for the complete degradation of gamma-chains. Fibrinogenolytic activity of natrahagin was 0.349 +/- 0.044 g.min-1.g-1 as determined by its ability to reduce the clottable fibrinogen. On the other hand, natrahagin concentration-dependently inhibited platelet aggregation induced by ristocetin in platelet-rich plasma and thrombin (80 U.L-1) in washed platelets with IC50 (95% confidence limit) of 56 (40-79) and 3.3 (1.4-8.0) mg.L-1. No inhibitory effect was found on collagen- and ADP-induced platelet aggregation even when the dose of natrahagin reached 200 mg.L-1.
CONCLUSION:
Natrahagin is an alpha, gamma-fibrinogenase with an inhibitory effect on platelet membrane glycoprotein Ib (GPIb)-dependent platelet aggregation.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8288}
}