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Inhibitory effect of dopamine on Ca(2+)-calmodulin-dependent protein kinase II activity in rat hippocampal slices.

  
@article{APS8272,
	author = {Xiao-yu Hou and Guang-yi Zhang},
	title = {Inhibitory effect of dopamine on Ca(2+)-calmodulin-dependent protein kinase II activity in rat hippocampal slices.},
	journal = {Acta Pharmacologica Sinica},
	volume = {20},
	number = {10},
	year = {2016},
	keywords = {},
	abstract = {AIM:
To study the effect of dopamine (DA) on Ca(2+)-calmodulin dependent protein kinase II (CCDPK II) activity in rat hippocampus.
METHODS:
Using rat hippocampal slices as an in vitro model, the activity of CCDPK II was examined by the method of 32P-incorporation.
RESULTS:
Exogenous DA reduced CCDPK II activity in hippocampal slices in a concentration- and time-dependent manner. Removal of extracellular calcium antagonized the DA-induced inhibition of CCDPK II activity, partially or completely. The activity of CCDPK II was markedly decreased by apomorphine (a nonselective DA receptor agonist), SKF38393 (a selective D1-like DA receptor agonist), or quinpirole (a selective D2-like DA receptor agonist). The inhibition of CCDPK II activity induced by exogenous DA was abolished by preincubation with Sch-23390, a selective D1-like DA receptor antagonist, or domperidone, a selective D2-like DA receptor antagonist.
CONCLUSION:
DA has an inhibitory effect on CCDPK II activity in rat hippocampus, related to stimulation of D1-like and D2-like receptors and calcium influx.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/8272}
}