@article{APS8269,
author = {Feng-qin Zhang and Zhi-pu Luo and Zheng-hua Gong},
title = {Desipramine and fluoxetine antagonized 5,7-dihydroxytryptamine-induced lesion on rat hippocampal and cortical neurons.},
journal = {Acta Pharmacologica Sinica},
volume = {20},
number = {10},
year = {2016},
keywords = {},
abstract = {AIM:
To assess the protective effect of desipramine (Des) and fluoxetine (Flu) on the neurons against the lesion induced by a selective serotonergic neurotoxin in vitro.
METHODS:
The 10-day cultured primary neurons of hippocampus and cortex of rat was exposed to 5,7-dihydroxytryptamine (5,7-DHT) to determine the optimal lesion concentration and duration. Before exposing to 5,7-DHT, Des and Flu was added to the medium for 30 min to observe the protective effects.
RESULTS:
The optimal concentration and duration for 5,7-DHT was 600 micromol.L-1 and 4 h, respectively. Both Des and Flu showed a protective effect in the dose range of 0.8 micromol.L-1 to 10 micromol.L-1 and 0.04 micromol.L-1 to 0.6 micromol.L-1, respectively, when the neurons were injured by 5,7-DHT 600 micromol.L-1 for 4 h. Flu showed a higher efficacy than Des. Both exhibited a more powerful protective effect on the hippocampal neuron than on the cortical neuron.
CONCLUSION:
The antidepressant effect of Des and Flu was attributed to their protective effect on the injured serotonergic neuron of the hippocampus and the cortex.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8269}
}