@article{APS8242,
author = {Fu-xian Yi and Wen-lan Liu and Li-wei Chen and Shan Zeng and Zhao-gui Guo},
title = {Hydrolysis of extracellular adenine nucleotides by cultured bovine endocardial endothelial cells.},
journal = {Acta Pharmacologica Sinica},
volume = {20},
number = {8},
year = {2016},
keywords = {},
abstract = {AIM:
To characterize the ATP diphosphohydrolase (apyrase) of bovine endocardial endothelial cells, and to compare ecto-adeninenucleotidase activity between bovine endocardial and aortic endothelial cells (BEEC and BAEC).
METHODS:
The nucleotide was analyzed by reversed phase HPLC and apyrase activity was assayed by inorganic phosphate release.
RESULTS:
Apyrase inhibitors, both NaN3 10 mmol.L-1 and NaF 20 mmol.L-1, inhibited BEEC apyrase activity by 51% and 38%, respectively. The inhibitor for Na+/K(+)-ATPase, ouabain, did not affect the enzyme activity. Edetic acid 5 mmol.L-1 completely inhibited the enzyme activity. H2O2 0.5 mmol.L-1 downregulated BEEC apyrase activity in a time-dependent manner. The apyrases activities in BAEC were higher than those in BEEC, while the ecto-AMPase activity in BAEC was much weaker than that in BEEC.
CONCLUSION:
BEEC have NaN3- and NaF-sensitive, ouabain-insensitive apyrase activity. BEEC had high ecto-AMPase activities, and low apyrases activities as compared with BAEC.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8242}
}