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Inhibition of histamine release from human mast cells by natural chymase inhibitors

  
@article{APS8223,
	author = {Shao-heng HE and Hua XIE and Xiao-jun ZHANG and Xian-jie WANG},
	title = {Inhibition of histamine release from human mast cells by natural chymase inhibitors},
	journal = {Acta Pharmacologica Sinica},
	volume = {25},
	number = {6},
	year = {2016},
	keywords = {},
	abstract = {AIM:
To investigate the ability of natural chymase inhibitors to modulate histamine release from human mast cells.
METHODS:
Enzymatically dispersed cells from human lung, tonsil, and skin were challenged with anti-IgE or calcium ionophore A23187 in the absence or presence of the natural chymase inhibitors secretory leukocyte protease inhibitor (SLPI) and alpha(1)-antitrypsin, then histamine release was determined.
RESULTS:
IgE-dependent histamine release from lung, tonsil, and skin mast cells were inhibited by up to 70 %, 61 %, and 62 %, respectively following incubation with alpha(1)-antitrypsin (5000 nmol/L). SLPI 5000 nmol/L was also able to inhibit anti-IgE-dependent histamine released from lung, tonsil and skin mast cells by up to approximately 72 %, 67 %, and 58 %, respectively. While neither alpha(1)-antitrypsin nor SLPI by themselves altered histamine release from lung, tonsil and skin mast cells, they were able to inhibit calcium ionophore-induced histamine release from lung and tonsil mast cells.
CONCLUSION:
Both micro(1)-antitrypsin and SLPI could potently inhibit IgE-dependent and calcium ionophore- induced histamine release from dispersed human lung, tonsil, and skin mast cells in a concentration-dependent manner, which suggested that they were likely to play a protective role in mast cell associated diseases including allergy.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/8223}
}