@article{APS8206,
author = {Lu-yang YU and Bo LIN and Zhen-lin ZHANG and Li-he GUO},
title = {Direct transfer of A20 gene into pancreas protected mice from streptozotocin-induced diabetes},
journal = {Acta Pharmacologica Sinica},
volume = {25},
number = {6},
year = {2016},
keywords = {},
abstract = {AIM:
To investigate the efficiency of transfer of A20 gene into pancreas against STZ-induced diabetes.
METHODS:
PVP-plasmid mixture was directly transferred into the pancreatic parenchyma 2 d before STZ injection. The uptake of plasmid pcDNA3-LacZ or pcDNA3-A20 was detected by PCR and the expression of LacZ was confirmed by histological analysis with X-gal. A20 expression in the pancreas of pcDNA3-A20 transgenic mice was measured by RT-PCR and Western blots. Urine amylase, NO generation, and histological examination were examined.
RESULTS:
Injection of PVP-plasmid mixture directly into the pancreatic parenchyma increased urine amylase concentration 16 h after operation and reversed it to nearly normal 36 h later. On d 33 LacZ expression could be found in spleen, duodenum, and islets. The development of diabetes was prevented by direct A20 gene transferring into the pancreas and A20-mediated protection was correlated with suppression of NO production. The insulitis was ameliorated in A20-treated mice.
CONCLUSION:
Injection of PVP-plasmid mixture directly into the pancreatic parenchyma led to target gene expression in islets. Direct transfer of A20 gene into the pancreas protected mice from STZ-induced diabetes.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8206}
}