@article{APS8187,
author = {Na LI and Ke-gui WU and Xiang-yu WANG and Liang-di XIE and Chang-sheng XU and Hua-jun WANG},
title = {Angiotensin II stimulates phosphorylation of 4E-binding protein 1 and p70 S6 kinase in cultured vascular smooth muscle cells},
journal = {Acta Pharmacologica Sinica},
volume = {25},
number = {5},
year = {2016},
keywords = {},
abstract = {AIM:
To examine the regulatory effects of angiotensin II (Ang II) on the phosphorylation of 4E-binding protein 1 (4E-BP1) and p70 S6 kinase in cultured vascular smooth muscle cells (VSMC), and the contribution of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (PKB) signaling pathway in this process.
METHODS:
VSMC obtained from rat thoracic aortas were cultured. The phosphorylation of 4E-BP1 and p70 S6 kinase was detected by immunoblotting.
RESULTS:
Ang II significantly increased the phosphorylation of 4E-BP1 and p70 S6 kinase, with the peaks occurring at, respectively, 10 min and 30 min, after stimulation with Ang II. The stimulatory effect of Ang II on 4E-BP1 and p70 S6 kinase phosphorylation was abrogated by Ang II type 1 receptor (A(T1) receptor) antagonist losartan, and suppressed by PI3K inhibitor LY294002 in a concentration-dependent manner.
CONCLUSION:
Ang II treatment of VSMC induces the phosphorylation of 4E-BP1 and p70 S6 kinase via A(T1) receptor, and PI3K signaling pathway is involved in this process.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8187}
}