@article{APS8186,
author = {Hai-Bo Qiu and Yi Yang and Shao-Xia Zhou},
title = {Effects of tripcholorolide on inflammatory reaction of mouse alveolar macrophages in vitro},
journal = {Acta Pharmacologica Sinica},
volume = {21},
number = {12},
year = {2016},
keywords = {},
abstract = {\"AIM:
To observe the effects of tripcholorolide (T4) on inflammatory reaction of mouse alveolar macrophages.
METHODS:
RT-PCR was used to investigate tumor necrosis factor alpha (TNF alpha), interleukin-1 beta (IL-1 beta), IL-6, IL-10, and inducible nitric-oxide synthase (iNOS) mRNA expression in alveolar macrophages after LPS 10 mg/L and T4 500 micrograms/L treatment. ELISA was used to detect TNF alpha, IL-1 beta, IL-6, and IL-10 protein expression. Nitrite was measured by Griess reaction.
RESULTS:
TNF alpha, IL-1 beta, IL-6, IL-10, and nitrite increased in supernatants, when alveolar macrophages were stimulated by LPS 10 mg/L at 24 h. Both T4 500 micrograms/L and dexamethasone 100 mumol/L had inhibitory effects on the production of TNF alpha, IL-1 beta, IL-6, IL-10, and nitric oxide. The mRNA expression of TNF alpha, IL-6, IL-10, and iNOS increased at 5 h after LPS stimulation which was decreased on addition of T4 500 micrograms/L or dexamethasone 100 mumol/L. T4 had no effect on stability of LPS-induced mRNA expression in TNF alpha, IL-6, and IL-10.
CONCLUSION:
T4 had inhibitory effects on the expression of proinflammatory and antiinflammatory mediators.\"},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8186}
}