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Oxidized LDL upregulated ATP binding cassette transporter-1 in THP-1 macrophages

   
@article{APS8159,
	author = {Chao-ke TANG and Guang-hui YI and Jun-hao YANG and Lu-shan LIU and Zuo WANG and Chang-geng RUAN and Yong-zong YANG},
	title = {Oxidized LDL upregulated ATP binding cassette transporter-1 in THP-1 macrophages},
	journal = {Acta Pharmacologica Sinica},
	volume = {25},
	number = {5},
	year = {2016},
	keywords = {},
	abstract = {AIM:
To study the effect of oxidized low density lipoprotein (ox-LDL) on ATP binding cassette transporter A1 (ABCA1) in THP-1 macrophages.
METHODS:
After exposing the cultured THP-1 macrophages to ox-LDL for different periods, cholesterol efflux was determined by FJ-2107P type liquid scintillator. ABCA1 mRNA and protein level were determined by reverse trancriptase-polymerase chain reaction (RT-PCR) and Western blot, respectively. The cholesterol level in THP-1 macrophage foam cells was detected by high performance liquid chromatography.
RESULTS:
ox-LDL elevated ABCA1 in both protein and mRNA levels and increased apolipoprotein (apo) A-I-mediated cholesterol efflux in a time- and dose-dependent manner. 22(R)-hydroxycholesterol and 9-cis-retinoic acid did significantly increase cholesterol efflux in THP-1 macrophage foam cells (P},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/8159}
}