@article{APS8148,
author = {Hong-yan CHEN and Huan-zhang ZHU and Bin LU and Xuan XU and Ji-hua YAO and Qi SHEN and Jiang-lun XUE},
title = {Enhancement of naked FIX minigene expression by chloroquine in mice},
journal = {Acta Pharmacologica Sinica},
volume = {25},
number = {5},
year = {2016},
keywords = {},
abstract = {AIM:
To study the effect of chloroquine on the expression of human clotting factor IX (hFIX) in mice.
METHODS:
Hydrodynamics-based naked DNA plasmid administration was performed by tail vein injection of 10 microg of pCMV- hFIX and chloroquine (0, 100, 200, and 500 micromol/L) in 2.2 mL of Ringer's solution within 6-7 s, the level and stability of hFIX expression, liver damage and toxicity were then examined.
RESULTS:
The maximum expression of hFIX level was 4.4+/-1.8 mg/L at 8 h after injection, 9.7+/-1.6 mg/L at 24 h only existed in 200 micromol/L chloroquine-treated animals, which is 3-4 fold higher than that of control (P0.05).
CONCLUSION:
Chloroquine can enhance and sustain exogenous gene expression in vivo without side effect under our experimental conditions.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8148}
}