@article{APS8147,
author = {Hua Wang and Yan-Ai Mei and Ming-Ming Wu and Jia-Ting Sun and Han-Qing Zhou and Zhi-Hong Zhang},
title = {Swelling-activated chloride currents in embryonic chick heart cells},
journal = {Acta Pharmacologica Sinica},
volume = {21},
number = {11},
year = {2016},
keywords = {},
abstract = {\"AIM:
To characterize a swelling-activated chloride current, I(Cl, swell), in white Leghorn chick heart cells and the effects of chlorpromazine (CPZ) effects.
METHODS:
The patch-clamp technique in the whole-cell configuration was used.
RESULTS:
Hyposmotic swelling elicited I(Cl, swell) in white Leghorn chick heart cells. The current amplitude increased from (452 +/- 200) pA to (849 +/- 373) pA with a reduction of osmolarity from 300 mmol.L-1 to 270 mmol.L-1, 4',4-Diisothiocyanostilbene-2,2'-disulphonic acid (DIDS) 100 mumol.L-1 decreased I(Cl, swell) from (1196 +/- 505) pA to (830 +/- 328) pA in hyposmotic solution. In white Leghorn chick heart cells I(Cl, swell) was not induced by CPZ 30 mumol.L-1, which is different from the case of E coli spheroplast.
CONCLUSION:
Swelling-activated chloride current was activated by hyposmotic swelling in white Leghorn chick heart cells. The mechanism for activating the current is different from that of mechanosensitive ion channels of E coli.\"},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8147}
}