@article{APS8143,
author = {Ying WANG and Jin-ming WANG and Shu-xun YAN and Ming-jiang LI and Jian-jun LI},
title = {Effect of angiotensin II receptor 1 antisense oligodoexynucleotides on physiological and pathophysiological growth of cardiomyocytes},
journal = {Acta Pharmacologica Sinica},
volume = {25},
number = {5},
year = {2016},
keywords = {},
abstract = {AIM:
To evaluate the role of angiotensin II receptor 1 antisense oligodexynucleotides (AT1R-AS-ODNs) on physiological and pathophysiological growth of cardiomyocytes from normotensive rats.
METHODS:
Cardiomyocytes were transfected with AT1R-AS-ODNs (200 nmol/L) followed by treatment with or without angiotensin II (1 micromol/L). In situ hybridization and Western blot were used for AT1R mRNA and protein detection, respectively. c-Jun N-terminal protein kinase (JNK) activity was characterized by immune complex kinase assay. c-Jun protein expression was examined by immunocytochemistry. DNA content was detected by flow cytometric assay. Atrial natriuretic factor (ANF) expression was identified by radioimmunoassay.
RESULTS:
Treatment with AT1R-AS-ODNs for 24 h resulted in 51.2 % decrease in AT1R mRNA and 60.7 % in protein (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8143}
}