@article{APS8136,
author = {Jun-Ying Miao and Satohiko Araki and Shang-Li Zhang and Hiroshi Hayashi},
title = {1-Methyl-3-isobutylxanthine delays apoptosis induced by deprivation of growth factors in vascular endothelial cells},
journal = {Acta Pharmacologica Sinica},
volume = {21},
number = {10},
year = {2016},
keywords = {},
abstract = {\"AIM:
To investigate the effects of 1-methyl-3-isobutylxanthine (MIBX) on apoptosis induced by deprivation of acidic fibroblast growth factor (aFGF) and serum in vascular endothelial cells (VEC).
METHODS:
Nuclear fragmentation was observed by fluorescence microscopy. Viability was determined by counting the cells that attached to dishes after treatments. DNA fragmentation was measured by agarose gel electrophoresis.
RESULTS:
The cells deprived of aFGF and serum were treated with MIBX 25-200 mumol/L for 3, 6, 9, and 12 h, respectively. Morphological changes including the formation of apoptotic bodies and DNA fragmentation of these cells were significantly suppressed by IBMX 50-200 mumol/L at 3 h and 6 h. But after 12-h treatment, no difference was observed between the treated and untreated cells.
CONCLUSION:
MIBX delays apoptosis of vascular endothelial cells induced by deprivation of aFGF and serum.\"},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8136}
}