@article{APS8114,
author = {Jian-Guo Zhuang and Yi Zhang and Zhao-Nian Zhou},
title = {Hypoxic preconditioning upregulates KATP channels through activation of protein kinase C in rat ventricular myocytes},
journal = {Acta Pharmacologica Sinica},
volume = {21},
number = {9},
year = {2016},
keywords = {},
abstract = {\"AIM:
To test: 1) if hypoxic preconditioning upregulates the activity of KATP channels in isolated adult rat cardiac myocytes; 2) if the upregulation involves protein kinase C (PKC).
METHODS:
By the whole-cell patch-clamp recording technique, KATP channel currents (IKATP) were measured in the cardiomyocytes with no pretreatment (CON), hypoxic exposure preceding reoxygenation (HPC), phorbol 12-myristate 13-acetate (PMA), and chelerythrine addition with HPC (CH + HPC), respectively.
RESULTS:
At 0 mV and 5 min after 2, 4-dinitrophenol (DNP) perfusion, the membrane currents for the CON, HPC, PMA, and CH + HPC were (3.5 +/- 1.9), (7.7 +/- 1.5), (7.5 +/- 3.3), (4.6 +/- 2.4) nA, respectively. Compared with the CON, the IKATP in the HPC and PMA were significantly augmented (P < 0.01) while the IKATP in the CH + HPC remained similar to the CON (P > 0.05).
CONCLUSION:
1) hypoxic preconditioning stimulated the activity of PKC and markedly enhanced the activity of KATP channels in the isolated rat cardiac myocytes; 2) PKC activation was involved in the upregulation of KATP channels.\"},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8114}
}