@article{APS8077,
author = {Qing ZHAO and Nan CHEN and Wei-ming WANG and Jian LU and Bing-bing DAI},
title = {Effect of transforming growth factor-beta on activity of connective tissue growth factor gene promoter in mouse NIH/3T3 fibroblasts},
journal = {Acta Pharmacologica Sinica},
volume = {25},
number = {4},
year = {2016},
keywords = {},
abstract = {AIM:
To investigate the regulatory mechanism of transforming growth factor-beta on activity of connective tissue growth factor promoter in mouse NIH/3T3 fibroblasts.
METHODS:
The regulation fragment of the 5' flanking region of the human CTGF gene was linked to pGL3-Basic vector, a firefly luciferase reporter construct without promoter. The recombinant plasmid pCTGF-luc was transiently transfected to NIH/3T3 fibroblasts. The activity of CTGF promoter after treatment of TGF-beta(1) and MAPK pathway inhibitors were assayed with luciferase reporter gene assay system.
RESULTS:
TGF-beta(1)-induced increase of CTGF promoter activity was concentration-dependent, with a plateau at 5 microg/L by 2.67-fold vs control (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8077}
}