@article{APS8042,
author = {Song-Lin Huang and Wei Wang and Dong-Sheng Ou-Yang and Hong-Hao Zhou},
title = {Determination of procaterol in human plasma by gas chromatography/electron impact ionization mass spectrometry},
journal = {Acta Pharmacologica Sinica},
volume = {21},
number = {6},
year = {2016},
keywords = {},
abstract = {\"AIM:
To improve a gas chromatography/electron impact ionization mass spectrometry (GC/MS) method for determining the concentration of procaterol in human plasma.
METHODS:
GC/MS was developed with capillary column. Samples were extracted by liquid phase before derivated. Imipramine was used as an internal standard. The injector and GC/MS interface temperatures were set at 280 degrees C and 250 degrees C, respectively. The carrier gas (helium) was 0.8 mL.min-1, and injections were made in the pulse-splitless mode. The MS source and MS Quad temperature were 230 degrees C and 150 degrees C, respectively.
RESULTS:
The detection limit of plasma procaterol was 5 ng.L-1. The assay was linear over the range of 10-10,000 ng.L-1 with correlation coefficient of 0.9987. The coefficients of variation were less than 10% for procaterol detection at high, medium and low concentration levels (n = 5). The average recovery of the assay was 99.1% +/- 1.3%.
CONCLUSION:
This assay was sensitive, precise, and accurate for evaluating the clinical pharmacokinetics of procaterol.\"},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8042}
}