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Phosphatidylinositol 3-kinase modulates IL-18-induced nuclear factor-kappa B activation

   
@article{APS7997,
	author = {Fu-Kun Guo and Shu-Guang Wu},
	title = {Phosphatidylinositol 3-kinase modulates IL-18-induced nuclear factor-kappa B activation},
	journal = {Acta Pharmacologica Sinica},
	volume = {21},
	number = {4},
	year = {2016},
	keywords = {},
	abstract = {\"AIM:
To investigate whether phosphatidylinositol (PI) 3-kinase is involved in interleukin-18 (IL-18)-induced nuclear factor-kappa B (NF-kappa B) activation.
METHODS:
Antisense PI 3-kinase oligonucleotide (ODN) was delivered by lipofectin encapsulation into cultured human hepatocellular carcinoma HepG2 cells. PI 3-kinase mRNA expression was assayed by semiquantitative reverse transcription-PCR. The levels of NF-kappa B were measured by sandwich ELISA.
RESULTS:
(1) Antisense PI 3-kinase ODN blocked PI 3-kinase mRNA expression. (2) IL-18 activated NF-kappa B from basal level of 0.153 +/- 0.008 to 1.942 +/- 0.017. (3) Antisense PI 3-kinase ODN inhibited IL-18-induced NF-kappa B activation in a concentration (1-8 mg/L)- and time (5-24 h)-dependent fashion. When the cells were treated with antisense PI 3-kinase ODN 2 mg/L for 8 h, a maximum inhibitory rate was 35.2% from 1.942 +/- 0.017 control to 1.259 +/- 0.018.
CONCLUSION:
PI 3-kinase is necessary for IL-18-stimulated NF-kappa B activation.\"},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/7997}
}